Beth Israel Deaconess Medical Center Research Assistant II in Boston, Massachusetts
Research Assistant II
Job LocationBoston, Massachusetts
Req ID 18237BR
Department Description: Candidate must be proficient in cell culture techniques (cell lines, primary cell cultures and iPSC-derived cells). S/he must have experience maintaining mouse lines (breeding, weaning, genotyping) and be comfortable with getting blood samples and performing injections. S/he must have experience with immunostaining of both paraffin-embedded tissues and cell cultures, confocal microscopy, cell harvesting, western immunoblotting, TUNEL assays, mRNA isolation and RT-PCR. Knowledge of cloning is required (generation of recombinant plasmids, site-directed mutagenesis, growth and titration of adenoviral solutions).Job Location: Boston, MAReq ID: 18237BRJob Summary: Under general supervision of the principal investigator, conducts a variety of routine and specialized laboratory tests using molecular, cell biology and/or biochemistry techniques. Performs lab procedures with speed, precision, and understanding of theory behind tests and procedures. Performs Level I responsibilities with a higher level of problem resolution, analytical ability, and self-direction.
Performs routine and non-routine laboratory tests (i.e.: DNA sequencing, gene cloning, in situ hybridization, immunohistochemistry, northern blotting) and synthesizes and interprets results.
May operate and maintain moderate to complex lab equipment such as spectrophotometer, flow cytometer, etc.
Using analytical abilities and sound judgment, makes decisions regarding the reliability and accuracy of results, repeating procedures when necessary.
May assist with planning specific research procedures and coordinating schedules of tests and procedures
May prepare written and/or verbal reports on status of research and the technical procedures used, including statistical and graphic summaries of findings and possible conclusions.
Bachelor's degree required in Life Sciences or related scientific field.
1-3 years related work experience required.
Bachelor's degree and one year of experience or a Masters degree with requisite knowledge and skills.
Experience with computer systems required, including web based applications and some Microsoft Office applications which may include Outlook, Word, Excel, PowerPoint or Access.
Written Communications: Ability to read, and write in English in order to understand basic safety instructions and take direction from supervisors; communicate effectively with patients, families and other medical center staff; and respond to basic questions.
Oral Communications: Ability to understand spoken English in order to follow basic safety instructions and take direction from supervisors; communicate effectively in basic English with patients, families and medical center staff in response to routine questions.
Knowledge: Ability to demonstrate basic knowledge of fundamental concepts, practices and procedures with the ability to use them in routine situations. Specialized knowledge not required.
Team Work: Ability to interact respectfully with other employees, professional staff and/or external contacts to offer ideas, identify issues, obtain information or deliver services.
Customer Service: Ability to demonstrate a positive attitude and respond to requests in a timely and respectful manner.
Physical Nature of the Job:
Light work: Exerting up to 20 pounds of force frequently to move objects. Some elements of the job are sedentary, but the employee will be required to stand for periods of time or move through out the hospital campus